Microscopy from Scientific Research

Fluorescence images of HIV-1 and SIVmac infection with Hoechst for nuclei and GFP for virus-infected cells in both normoxia and hypoxia. The number of GFP-positive cells was quantitated and normalized to the total number of cells to give a single-round infection rate. The rates were then normalized with those in normoxia conditions for either HIV-1 or SIVmac infection. Duplicate infections per donor are plotted for each donor in each condition (n= 34). One-sample t test was used for statistical analyses, with error bars representing SEM and*p< 0.5. The scale bar represents 100 m.

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