t/f-clusters are significantly separated in anatomical tectal space within t-types and f-types. Left, pairwise KDE overlap values for cell types of different functional clusters for real data and shuffled t-type labels. Right, as left but for different t-types within functional clusters. Mean pairwise overlap values of t/f-clusters across t-types and across f-types are significantly lower than respective shuffled controls, respectively, indicating anatomical separation of cell types within functional clusters. Two-sided MannWhitney U -test, Bonferroni-corrected. P values: t-types overall, 3.353 x 10 -22 ; atf5b , 5.598 x 10 -11 ; itpr1b , 2.203 x 10 -4 ; pitx2 , 9.370 x 10 -1 ; sp5l , 2.662 x 10 -9 ; clusters overall, 2.230 x 10 -14 ; c6, 1.822 x 10 -7 ; c7, 2.641 x 10 -4 ; c14, 4.032 x 10 -3 ; c16, 3.463 x 10 -2 .
