ALDH1A3 cellular localization and levels. Immunocytochemistry (ICC) staining by fluorescence antibody labeling ALDH1A3 in A375 sorted ALDHHigh and ALDHLow cells. Scale bar, 50 μm. Quantification of cells enriched with nuclear ALDH1A3: experiment repeat n= 2, quantified image fields: 11 for ALDHHigh and 9 for ALDHLow, mean ± SD; non-paired Kolmogorov-Smirnov test, ∗∗∗∗p< 0.0001. Cells showing minimal cytoplastic content outside DAPI-stained regions (indicated with an asterisk [∗]) were determined as unfit following FACS and thus excluded from quantification.
