Summary plots of peak levels of HA and PSD-95 at side-view synapses stained for HA (STED), PSD-95 (STED), and synapsin (confocal). CaV2 cTKO, 200/3; CaV2 cTKO + CaV2.1, 180/3; CaV2 cTKO + CaV2.1ΔEF1, 203/3. Data are mean ± SEM;∗∗∗p< 0.001. Statistical significance compared to CaV2 cTKO was determined with Kruskal-Wallis tests followed by Dunn’s multiple comparisons post hoc tests for the protein of interest.