Microscopy from Scientific Research

Multiplexed in situ HCR of selected marker genes were registered to the standard brain atlas at mapzebrain.org. We measured expression level by examining the pixel intensity profile of each gene in the same transverse (top, projection of planes z289–z291) and coronal (left, projection of planes x463–x465) section. Left, the area in which pixel intensity is measured is indicated with a white dashed rectangle and confined to the SPV (S, superficial; D, deep). Middle and right, expression pattern example in a single fish.

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