Microscopy from Scientific Research

Mouse Neuro-2a cells were transiently transfected with WT mCherry-MAGEL2 or mCherry-p.Gln666Profs*47 (magenta) fusion proteins and subcellular localization was analyzed employing confocal immunofluorescence microscopy 24 h after transfection. Nuclei were counterstained with DAPI (blue). FMRP was visualized with anti-FMRP antibodies (green). A white signal in the overlap images indicated signal overlap or co-localization. At least 3 transfected cells were imaged in three independent replicates and representative cells are depicted. The scale bar is 10 m.

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