Microscopy from Scientific Research

Confocal immunofluorescence microscopy of recombinant fusion proteins WT mCherry-MAGEL2 or mCherry-p.Gln666Profs*47 (magenta) in transiently transfected HEK293T cells, 24 h after transfection. Nuclei were counterstained with DAPI (blue). The scale bar is 10 μm. White arrows indicate sites of spatial proximity between WT MAGEL2 and p.Gln666Profs*47 with recycling endosomes, which were visualized with anti-RAB11A antibodies (green). At least 5 cells were imaged in three independent replicates and representative cells are depicted.

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