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Top: a diagram of the tissue-specific Cas9 construct designed to locally disrupt YDA in abscission zone cells using the HAE promoter. Bottom: the gene model of YDA where boxes represent exons, a red arrow marks the target site for the Cas9 nuclease. Underneath, the floral organ abscission profile of the ydaTSKO line with the strongest impairment is shown to highlight the potential to impair abscission by disrupting YDA in AZ cells. Scale bars, 1 cm. See alsoFigure S3I.

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