β-galactosidase activity assay in BTH101E. colicells expressing T18 and T25 fusions toS. oneidensisFlaB, full-length Maf-1, truncated Maf-1 variants (Maf-11–420and Maf-11–699) and CTD variants of Maf-1 (CTD421–822and CTD550–822). Unfused FlaB, full-length Maf-1, truncated Maf-1, and CTD variants as well as empty plasmids and BTH101 cells were included as negative controls. Except for two combinations (T18-CTD421–822/T25-FlaB and T18-CTD421–822/T25-empty) which gave only one co-transformant, β-galactosidase activity of each combination was measured from a culture with five pooledE. coliclones grown overnight in LB containing antibiotic (when appropriate) and IPTG.
