Gel from Scientific Research

Open access visualization of Gel, Microscopy, TA Toxicity Assays, TAC Toxicity Assays, E. coli BW25113
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DOI: 10.1016/j.chom.2024.05.003

DOI

TA and TAC toxicity assays inE. coliBW25113 protease-deficient strains. Indicative of antitoxin degradation by ClpXP, the CmdTA TA unit is non-toxic in clpP and clpX but not lon strains (left). The CmdTA TA toxicity is abrogated upon the Asp-Asp substitution of the C-terminal Ala-Ala motif of CmdA that is recognized by ClpX.

#Gel#Microscopy#TA Toxicity Assays#TAC Toxicity Assays#E. coli BW25113#Protease-deficient Strains#Antitoxin Degradation#ClpXP#CmdTA TA Unit#ClpP#ClpX#Lon#Asp-Asp Substitution#Ala-Ala Motif#CmdA

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