Protein Structure from Scientific Research

Structures of Cas12a showing the lower back half of the protein during R-loop propagation. Protein domains are shown as the reconstruction maps, and nucleic acids are shown as models. WED and PI domains are removed for clarity. REC2 becomes dislodged as the R-loop extends past the seed and then returns to a cleavage-competent conformation after R-loop reaches 16 bp. BH domain flexibility is also seen.

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