Line Plot from Scientific Research

Competition cleavage assay to test the ability of R-loop intermediate structures to stably bind Cas12a and inhibit cleavage of the matched PT DNA. Competitor DNAs were pre-incubated with Cas12a (variable times noted) before addition of labeled PT DNA. Plot shows competition time courses of NTS cleavage of the PT substrate. In (B) and (D), example cleavage reactions with six time points are fit to a single exponential curve (not all time points are shown). Only a single replicate is shown for each condition. See alsoFigure S3.

DOI