Effect of DnaJ on NC1-646conformation. Top: difference in deuterium uptake, after 10 or 100 s deuteration, between NC peptides in RNC1-646with or without bound DnaJ. Lower values indicate less deuteration of peptides in DnaJ-bound RNC1-646relative to free RNC1-646. Bottom: -gal monomer structure truncated after 626 residues, with protected regions (>=0.5 Da) colored blue, sites detected in DnaJ peptide array colored green, and residues that crosslink to DnaJ shown as orange spheres. Regions that were not covered in the HDX-MS experiment are colored dark gray.
