Effect of DnaK on NC conformation. Top: difference in deuterium uptake, after 10 or 100 s deuteration, between NC peptides in RNC1–646with or without bound DnaK. Lower values indicate less deuteration of peptides in DnaK-bound RNC1–646. Bottom: regions showing changes in deuterium uptake >=0.5 Da at either time point are mapped onto the structure of beta-gal truncated after 626 residues. Residues on RNC1–646that crosslink to DnaK are shown as orange spheres. Regions that were not covered in the experiment are colored dark gray. See alsoFigures S3, S8, and S9andTables S5andS7–S9.
