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TF binding to mutated RNCs. Coomassie-stained SDS-PAGE of the resuspended ribosomal pellet from high-salt co-sedimentation assays of RNCs, purified from WTE. coli, and incubated with additional TFin vitro. Bands corresponding to NCs (*) and TF are indicated. Immunoblots against TF are shown below. Positions of mutations are shown in red on the domain structure of NCs.

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