Bar Plot from Scientific Research

Electroporation efficiency of plasmid pJV420 subjected to IMPRINT with different MTase combinations into B. breveUCC2003. All transformation efficiencies are normalized to that of unmethylated DNA, which had a mean transformation efficiency of 3.63 x 105 CFUs/μg DNA. Dots represent transformation fold increases from independent experiments starting from separate TXTL reactions. Bars represent the geometric mean of the dots. *p< 0.01, *p< 0.05, ns:p> 0.05. HM, host-methylated plasmid DNA extracted from B. breveUCC2003 and subjected to IMPRINT without any added MTases.

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