Electroporation efficiency of plasmid pJV400 subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA, which had a mean transformation efficiency of 2.09 x 104 CFUs/μg DNA. Dots represent transformation fold increases from independent experiments starting from separate TXTL reactions. Bars represent the geometric mean of the dots.*p< 0.01,*p< 0.05, ns:p> 0.05. HM, host-methylated plasmid DNA extracted from S. enterica LT2 and subjected to IMPRINT without any added MTases. See also Figures S2 and S3.
