Bar Plot from Scientific Research

Electroporation efficiency of plasmids pJV400 (top) and pJV414 (bottom) subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA, which had a mean transformation efficiency of 1.27 x 104 colony-forming units (CFUs)/μg DNA (pJV400) and 1.46 x 103 CFUs/μg DNA (pJV414). Dots represent transformation fold increases from independent experiments starting from separate TXTL reactions. Bars represent the geometric mean of the dots.*p< 0.01,*p< 0.05, ns:p> 0.05. HM, host-methylated plasmid DNA extracted from S. enterica LT2 and subjected to IMPRINT without any added MTases. See also Figure S1.

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