Microscopy from Scientific Research

HK1-rings prevent FCCP-induced mitochondrial fission during energy stress. Thresholded images of mitoDsRed signal in HeLa cells (top) and in HeLa cells that overexpress HK1-GFP (bottom). Glucose was depleted for 30 min before imaging (leftmost images). The middle images show the cells after perfusion with 2 M FCCP for 20 min. Numbered dashed squares are magnified on the right side of the overview images. White arrows indicate mitochondrial fission and yellow arrows indicate mitochondrial constriction. The beeswarm SuperPlot represents each cell with a color-coded dot according to the experimental day (right). Two-tailed paired t test was used for statistical analysis (n= 3). Data are presented as mean SD.

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