Percentage of CD69+(early activation) and CD25+(late activation) CAR-T cells within the CD8+and CD4+subset after 24 h of dasatinib (50 nM) treatment either in the presence of MDA-MB-231 cells (effector-to-target cell ratio, 10:1) or without, cultured in a well-plate format. Data were acquired from flow cytometry analysis for both (A) and (B);n= 4 wells. Data are depicted as mean with SEM. Each red dot represents one well. ns, not significant,****p< 0.0001; Tukeys multiple comparisons test.
