Representative images of MDA-MB-231 aggregates (cyan) on day 8 after tumor-on-chip perfusion with CAR-T cells (red), showing different degrees of T cell infiltration into the aggregate after applying different dasatinib treatment schemes compared with an image of untreated cells. Conditions are indicated at the top left of each image. The yellow dashed line marks the region of interest of one tumor aggregate. Scale bars, 200 m.