Microscopy from Scientific Research

Immunostaining for indicated markers on HNES1 GATA3:mKO2 cells following 1 day of treatment with PD03+A83 and release into N2B27 for 16 h. Scale bars, 50 M. Zoomed-in inset scale bars, 5 M. Quantification of GATA6/NANOG double-positive cells as a percentage of total NANOG-positive cells in 5 independent fields of view. Data are mean with standard deviation (SD),n= 3,305 nuclei.

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