Cell clustering and differential gene expression analysis of scRNA-seq data from UTUC and NAT tissues. (A) UMAP visualization of 16 distinct cell clusters identified in UTUC and NAT tissues. (B) Dot plot showing the expression of marker genes in each cell cluster. The size of the dot represents the proportion of cells expressing the gene, and the color intensity represents the average expression level. (C) Stacked bar plot showing the proportion of each cell type in UTUC and NAT tissues. (D) Heatmap showing the differentially expressed genes (DEGs) between UTUC and NAT tissues in each cell cluster. (E) GO enrichment analysis of DEGs in tumor cells. (F) KEGG pathway enrichment analysis of DEGs in tumor cells. (G) GSEA analysis of DEGs in tumor cells.