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Immunoblot analysis with the indicated antibodies of FADD immunoprecipitates and total lysates from primary WT and Ripk1R588E/R588E MEFs stimulated with T (20 ng mL−1), C (1 μg mL−1), and zVAD (20 μM) for the indicated periods of time. Representative data shown from two independent experiments. Scale bars, 100 μm. Each dot represents one mouse. Graphs show mean ± SEM.*p≤ 0.05;**p≤ 0.01;***p≤ 0.001;****p≤ 0.0001 for all figures. For gel source data, see supplemental information. See also Figure S1.

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