Microscopy from Scientific Research

Top row: hematoxylin and eosin stain of the cerebellum with neuronal loss in the granule cell layer (GCL), vacuolation in the white matter (WM), and enlarged vessels/aneurysms (asterisks) observable in GIFN-FL mice (scale bars, 100 m; ML, molecular layer). Middle row: neurofilament immunohistochemistry shows Purkinje neurons (arrowheads) and their absence in GIFN-FL mice (asterisk; scale bars, 30 m). Bottom row: calcification in the GCL revealed with alizarin red S only in GIFN-FL mice (arrow: clustered calcified deposits in the GCL; scale bars, 100 m;n= 4 per genotype at 16 weeks of age). Representative images from two independent experiments.

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