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Mice were injected intraperitoneally (i.p.) with Evans blue, with overnight circulation, followed by fluorescein injection, and then perfused after 30 min. Evans blue and fluorescein permeate a leaky brain (n= 5 for WT-FL and GIT and 4 for GIFN-FL at 16 weeks of age; black lines are 1 cm apart and bisected by gray lines).

DOI