Representative confocal images of MTFP1-KO U2OS cells stably expressing Flag-MTFP1, pre-treated with the indicated siRNAs, MitoParaquat (MitoPQ) (for 30 min) or CCCP (for 1 h) and incubated with 25 μM CQ for 6 h. White arrows indicate the presence of SMEM within autolysosomal structures. Scale bars, 10 μm and 2 μm.
