Representative confocal images of Flag-MTFP1 or Flag-MTFP1mLIR x3overexpressing U2OS cells incubated in the presence or absence of CQ, showing the presence of SMEM (white arrows) within autophagosomes. Scale bars, 10 m and 2 m.n= 4 independent experiments. CQ treatment: 25 M for 6 h.
