Quantification of the mitochondrial area marked by OCT-PA-GFP probe 20 s after photoactivation (Q) and probe diffusion, at time 0 compared to 20 s and at time 20 s compared to 10 min (R), from (P).n= 4 independent experiments. All values: mean SD, except (F) and (N): mean SEM;n= 3 independent experiments, otherwise specified. (C): two-way ANOVA, Tukeys multiple comparison test; (D), (I), and (L): nested ordinary one-way ANOVA, Tukeys multiple comparisons test; (F), (N), (Q), and (R): non-parametric Mann-Whitney test; (H): nested t test (two-tailed).p< 0.05;p< 0.01;p< 0.001;p< 0.0001; nsp> 0.05. See alsoFigures S1S3.