Pharmacological potentiation and inhibition of iodide flux rates in CFBE41o−cells homozygous for ΔF508 CFTR. 1 μM lumacaftor was used in the culture to facilitate surface expression. A pre-treatment with 20 μM forskolin was used to activate ΔF508 CFTR. The relative potentiation was calculated as the ratio of flux rates with and without modulator at the indicated concentrations. Data points represent the means and SEs of 2 (CFTRinh-172) or 3 (all other conditions) experiments. Statistical significance relative to a DMSO-only treatment was tested by one-way analysis of variance (ANOVA) (*p= 0.049 for I1412,p= 0.041 for I1422, andp= 0.015 for CFTRinh-172;****p= 9.0x10−7for GLPG-1837,p= 7.8x10−6for Ivacaftor,p= 3.0x10−6for I1421,p= 1.0x10−5for I1408,p= 6.1x10−7for (S)-SX263, andp= 3.1x10−6for BBG3).
