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A schematic depiction illustrating the DID paradigm and Abx treatment. The light gray horizontal bar represents the 20% v/v ethanol drinking for 2 h during the first 3 days and for 4 h on the fourth day. The light orange horizontal bar symbolizes the administration of antibiotics (Abx) or PBS by oral gavage. The light blue horizontal bar represents the rest day post-antibiotic treatment. The black arrow represents the measurement of ethanol consumption and BEC at the completion of the 4 h of ethanol drinking.

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