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CD4 + T cells were cultured under Th17 polarizing condition, in the presence or absence of C3 (1 mM) or TSA (0.5 μM), collected at 24 h, and analyzed for HDAC activity using an HDAC activity assay kit at a fluorescence intensity of excitation/emission (490/525 nm). Data are expressed as mean ± SEM ( n = 3 each group), ** p < 0.01 and *** p < 0.001, **** p < 0.0001, determined by one-way ANOVA.

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