EA promoted Alloprevotella rava growth and inhibited Th17 polarization by regulating protein acetylation. a EA (5 mg/ml) co-cultured with Alloprevotella rava in vitro. b Number of colonies growing in the per field of in vitro co-cultivation of sterile water, EA, and Alloprevotella rava . c Concentration of C3 produced by in vitro co-culture of sterile water, EA, and Alloprevotella rava . CD4 + cells were isolated from spleen of nave C57BL/6 mice and cultured under Th17 polarizing conditions for 72 h under vehicle or EA treatment. d - e CD4 + T cells were treated with vehicle or EA for 72 h under the Th17 condition. IL-17 + production was analyzed by flow cytometry. f CD4 + T cells were treated with vehicle or EA under the Th17 condition for 24 h. The effect on protein acetylation expression in Th17 cells was analyzed by Western blot. * p < 0.05, ** p < 0.01 and **** p < 0.0001. The same letter represents NO significant difference between the two groups. Data are expressed as mean SEM ( n = 5 each group), determined by unpaired Student's t -test (b, c), and one-way ANOVA ( e ). One representative of three independent experiments is shown
