Line Plot from Scientific Research

Competition and Colony formation assays performed in chr8p non-deleted SNU387 liver cancer cell lines using two independent sgRNAs to target MFRN2. sgRNAs against essential genes KPNB1 and PCNA (gray) were used as positive controls, while a non-targeting sgCTR (black) was used as a neutral control. Competition assay results are shown as the mean SD of the ratio of GFP + cells compared to t0 for each assay using FACS analyses over 12 days with measurement at indicated time points. n = 3 biological replicates. Colony formation assays are depicted with representative technical triplicates of cells plated into 12-well plates and grown for 12 days n = 3 biological replicates.

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