Doxycycline (Dox) inducible differentiation of mouse ESCs into neurons. Top , RT-qPCR analysis of differentiation stage-specific markers in Dox-induced TRE-Ngn2 samples. Data are averaged from 3 experiments with SD shown by the shaded areas. Bottom , Dox-induced TRE-Ngn2 cells were treated for 6 h with DMSO or CHX and analyzed by RNA-seq.