Deconvolution results for simulated mixtures of 25 cell types, with the left-most cell type having a ground truth proportion of 0.00308 (red circle), and each cell type increasing linearly by 0.00308 until the right-most cell type, which has a proportion of 0.07692 (the increment is set to 0.00308 or 1/325 so they sum to 1; see the Methods section). The deconvolution estimates for each cell type is shown in a gray box plot: the boxes represent the first to third quartiles of 50 independent simulations, with whiskers extending up to 1.5 times this interquartile range. Outliers are denoted by dots. Each deconvolution model is shown separately, with a read length of 5 (left column) and 1 (right column). The Normalized Root Mean Square Error (NMRSE) and NRMSE standard deviation are shown for each.