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Activity of ecCFAS on different phospholipids and mono-CP/di-CP products ratio. All the reactions were carried out with 5 uM ecCFAS, 5 uM SAH-N, phospholipid 2 mM, SAM 4 mM in 100 mM HEPES buffer pH 7.5, 150 mM NaHCO3, 37 °C, 300 rpm, 100 uL reaction volume. Lipids were prepared by extrusion to 100 nm. PE 18 : 1 was co-solubilised with PC 14 : 0 (PE18 : 1/PC14 : 0 4 : 1) to overcome the low PE solubility in buffer.

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