Heatmaps represent the detail of DAPs (Students t test; FDRp <0.05) in buds (IB) and pollen (IP) after IMTACT (for corresponding gene description, seeData S4; LFQ intensities). Colored bars on the right of the heatmaps: significantly more abundant in pollen (pink) or in buds (beige). Categories were isolated from LFQ transformation, averaging, and filtering (n =388). Details about the subcellular localization analysis inFigures S6andS7. Analysis of the LFQ filter dataset (Data S4A) is detailed inFigures S8andS9. (A–D) (A) Protein metabolism/translation (Bin29;n =77), (B) RNA metabolism (Bin27;n =45), (C) TCA (Bin8;n =23), and (D) ETC (Bin9;n =45). Details on the TCA cycle are shown inFigure S10. Photorespiration, AA metabolism, transport, miscellaneous, and not assigned-related DAPs detected by MS are shown inFigures S11andS12andData S6. In blue: the lowest protein level in a defined functional category. In red: the highest protein level in a defined functional category. In gray: proteins not detected by MS in any of the four replicates. AOX, alternative oxidase; cytc, cytochrome c; DH, dehydrogenase; Misc., miscellaneous; MORFs, multiple organellar RNA editing factor; N.d., not detected; OGDH, 2-oxoglutarate dehydrogenase; PPR, pentatricopeptide repeat; PTM, post-translational modification; RPPR, ribosomal PPR; Ubi, ubiquitin. See alsoData S4andS6.
