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Fluorescence intensity at 428 nm for positions 1–5 as a function of time after the injection of 1 μL of a 60 mM stock solution of1in the center of a gel containing DPH (2.5 μM), λex/λem=355/428, slits=5/10. Experimental conditions: agarose=1 mg/ml, [HEPES buffer]=5 mM,T=25 °C. Error bars indicate the standard deviation calculated from duplo measurements.
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