Boxplots represent the amount of injection volume tolerated in the acute stretch paradigm by HU-treatede-cadherinmorphant embryos along with appropriate controls (G) and the control andNp63morphant embryos injected withCAAX-mCherryande-cadherin-mCherryRNA (J). Quantification and statistical analyses were performed usingn= the total number of embryos collected from multiple sets, as indicated below graphs (e, embryos). In the boxplots, center lines depict medians, while box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; notches in the boxplot are defined as 1.58 IQR/sqrt(n). hpi, hours post-injection; NS, no stretch; S, stretched.p< 0.05,p< 0.01, andp< 0.001 by Kruskal-Wallis test with Dunn’s post hoc test. We considerp= 0.051 as a statistically significant difference.
