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Schematic for preparation of spherical supported lipid bilayers (SSLBs) from liposomes. A lipid mix of desired composition (only PC or PC + 5 mol % PA) is dried into a lipid film and hydrated to obtain liposomes. Liposomes are then incubated with latex beads to form SSLBs. Buoyant SSLBs are separated by centrifugation and incubated with ATP releasate to recruit motors and other proteins onto the SSLB membrane. SSLBs are then introduced into a flow chamber having microtubules adhered to the cover slip surface for observing free SSLB motion or force generation by SSLB-bound motors against an optical trap (focused laser beam).

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