A representative intrinsic optical signal imaging (IOSI) image showing the location of the barrels (false-colored in light gray) corresponding to the whiskers stimulated during the procedure (yellow shading at the center of the image) in one mouse. A projection of the two-photon imaging FOV acquired throughout learning is overlaid on the IOSI image. Scale bar, 200 m, indicating anterior (A) and medial (M) directions.
