Screening of select sera (n= 66) for neutralization of RVPs corresponding to TBEV (European subtype, D). Shown is the rank-ordered luciferase activity relative to “no-serum” control (lower values correspond to higher neutralization). Each bar represents an individual participant sample analyzed at 1:1,200 dilution; average reading of duplicate wells. In (A)–(D): black, dark gray, and light gray represent samples from New York, Brazil, and Mexico, respectively. In red is MEX58 and in blue a sample from a TBEV-vaccinated individual. The p-values in (A)–(D) were determined with the Mann-Whitney test.
