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Comparison of Pearson’s correlation coefficient (PCC) of ANO4 and pan-cadherin positive pixels from immunostainings of HEK293 cells expressing ANO4 wild-type or mutants Met563Lys (M563K), Asn558Lys (N558K), Ile562Phe (I562F), Asn603Asp (N603D), Asn129Lys (N129K), Val528Met (V528M), or Ile725Thr (I725T). Values are given as mean ± SEM. Multiple comparisons were performed by ANOVA with Dunn’s post hoc test. Data points that were statistical outliers were eliminated by the Grubbs outlier test.*p< 0.05;****p< 0.0001; ns, not significant;nis given in parentheses; in (B),ngives the number of analyzed cells from three independent experiments.

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