Voltage dependences of peak conductance activation (circles) and steady-state inactivation (squares) are shown for Kv4.1 wild-type (WT), p.Arg92Cys, p.Asp115Asn, and p.Lys450*ternary channels. For the study of steady-state inactivation, brief control and test pulses to +40 mV were separated by a 10 s conditioning pulse (Vbetween -100 and 0 mV in 10 mV increments, see inset). Normalized data (Itest/Icontrol) were plotted against the conditioning pulse voltage. For the study of peak conductance activation, test pulses to voltages between -80 and +60 mV (Vin 10 mV increments) were applied from -100 mV (see inset). Normalized conductance values were plotted against the test pulse voltage. The data were fitted with appropriate Boltzmann functions.11,36Gray symbols: Kv4.1 WT; black symbols: data which do not differ from Kv4.1 WT; red symbols: data that differ from Kv4.1 WT (error bars are SEM).
