Microscopy from Scientific Research

Open access visualization of Microscopy, Thapsigargin, ER, Cytosolic Calcium, Mechanostimulation
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DOI: 10.1016/j.devcel.2024.03.014

DOI

With thapsigargin (Tg) treatment depleting ER Ca2+, the cytosolic Ca2+level did not increase after mechanostimulation as indicated by GCaMP6 intensity. 3 μM Tg was added to cells and incubated for 30 min in normal cell culture medium without EDTA before blue light stimulation.

#Microscopy#Thapsigargin#ER#Cytosolic Calcium#Mechanostimulation#GCaMP6 intensity

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