Confocal images showing orthogonal planes of control- and Oct4-depleted embryoids described in (C). Filled arrowheads point at continuous, thickened laminin associated with Oct4-positive cells, open arrowheads indicate discontinuous, thin-layered laminin associated with Oct4-depleted cells. The cartoons in (A), (C), and (F) illustrate the analyzed developmental stage and include auxillary details on image acquisition and presentation. Scale bars, 50 μm (left) and 10 μm (right). See alsoFigures S4andS5.
