Density Plot from Scientific Research

ALG2 CRISPR knockout U2OS cell stably expressing pHluorin-mKate2-SEC23 treated with 250 nM Torin for 6 h. Arrowheads point to a mKate2-labeled SEC23 signal inside of a LAMP1-positive structure. Note that pHluorin-labeled SEC23 is quenched in the LAMP1-positive structures. Scale bar: 1 μm. Quantification of pHluorin or mKate2 intensities from dsRed-pHluorin-SEC23 stably expressing cells after being treated with 250 nM Torin for 0, 4, 8 h as measured by flow cytometry (right panel). Mean fluorescence intensity (MFI) was quantified; dashed lines were drawn at the intensity mode at 0 h.

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