U2OS cells expressing LAMP1-HaloTag, CHMP4b-mNeonGreen, mScarlet-ALIX (upper panel), ALIX Bro1 domain truncation (middle panel), or ALIX PPR domain truncation (bottom panel) treated with 250 nM Torin for 6 h. The right panel shows a line scan of relative CHMP4b, ALIX, and LAMP1 intensities from arrowhead-pointed signals in the correlative images. Arrowheads point to LAMP1-positive structures colocalized (ALIX WT and PPR domain truncation-expressing cell) or not colocalized (ALIX Bro1 domain truncation-expressing cell) with CHMP4b and ALIX. Scale bar: 1 m.
