U2OS cells expressing mOrange2-SEC23, LAMP1-HaloTag, mEmerald-ALG2, or mEmerald-ALG2 E47A/E114A mutant were treated with 25 uM ML-SA1 for 10 min. The images show no treatment (upper), after ML-SA1 treatment (middle), and cells expressing ALG2 mutant after ML-SA1 treatment (bottom). Scale bar: 1 um.
